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Vegetos- An International Journal of Plant Research
Year : 2017, Volume : 30, Issue : 4
First page : ( 75) Last page : ( 80)
Print ISSN : 0970-4078. Online ISSN : 2229–4473.
Article DOI : 10.5958/2229–4473.2017.00194.X

Effect of Osmotic Stress on the Surface Properties of Protoplasts as Measured by Merocyanine 540

Sain M Pablo1,2, Rodríguez Silvia2, Disalvo A Edgardo1

1Laboratorio de Biointerfases y Sistemas Biomiméticos, Centro de Investigaciones y Transferencia de Santiago del Estero (UNSE-CONICET), RN 9, Km 1125, Villa El Zanjon, CP 4206, Santiago del Estero, Argentina

2Laboratorio de Ciencia y Tecnología de Alimentos, Centro de Investigaciones y Transferencia de Santiago del Estero (UNSE-CONICET), RN 9, Km 1125, Villa El Zanjon, CP 4206, Santiago del Estero, Argentina

*Corresponding author: Edgardo A Disalvo, Laboratorio de Biointerfases y Sistemas Biomiméticos, E-mail: disalvoanibal@yahoo.com.ar, pablosain28@hotmail.com, silviadepece@hotmail.com

Online published on 6 January, 2018.

Abstract

Protoplasts isolated from arugula leaves show the appearance of hydrophobic regions in the surface when cell volume is increased as detected using by Merocyanine 540 (MC540).

The analysis of the equilibrium of the dye species indicates that MC540 monomers and dimers distribution varies at each osmotic stage. It is suggested that this is a consequence of the changes produced in the surface packing at the membrane level, due to the affinity of this dye with lipid environments, which could be important for the interaction of enzymes and other solutes produced by plants during hydric stress.

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Keywords

Osmotic stress, Protoplast, Interfacial properties, Merocyanine 540.

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