Establishment of a highly efficient regeneration system for the mature embryo culture of tall fescue
*Corresponding author: Limei Gao, Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, PR China, E-mail: email@example.com
In this study, the effects of some factors including inoculation methods, initiating culture media, organic additives, antioxidants, and auxins on the regeneration of explants were evaluated by using the mature embryos derived from tall fescue (Festuca arundinacea Schreb. Houndog5). The results indicated that the sliced mature seeds culture was better than the whole mature seeds culture, the MD medium was better for callus induction than other mediums, and the callus induction frequency is the highest in MD medium supplemented with 9.0 mg L−1 of 2,4-D. A new MDW medium was established in this study by adding cysteine, ascorbic acid, glutamine into MS medium consisting of 0.5 mg L−1 2,4-D at the concentration of 40, 100 and 5 mg L−1, respectively, for callus propagation. By using the MDW medium and the sliced technique, the regeneration frequencies of embryonic callus became 88.6%, which was about 3 times higher than that obtained directly from MD mediums and the conventional culture techniques. This novel regeneration system could be helpful in tall fescue tissue culture and gene transformation.
Mature embryo, Tall fescue, Callus induction, Regeneration system.