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Vegetos- An International Journal of Plant Research
Year : 2015, Volume : 28, Issue : 2
First page : ( 90) Last page : ( 98)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.
Article DOI : 10.5958/2229-4473.2015.00042.7

Effect of a Potent Cytokinin Thidiazuron (TDH) on the Regener-ation Potential of Leaf and Petiole Explants of Cauliflower (Brassica oleracea L var. botrytis)

Gaur Ayesh*, Kumar Pankaj, Srivastava D. K.

Department of Biotechnology, Dr. Y. S. Parmar University of Horticulture and Forestry, Solan-173230, Himachal Pradesh, India

*Corresponding author E-mail: ayesh.gaur@gmail.com, dksuhf89@gmail.com

Online published on 7 October, 2015.

Abstract

Plant regeneration studies were carried out using leaf and petiole explants on forty dif-ferent combinations and concentrations of TDZ i. e. TDZ, TDZ+IAA, TDZ+NAA and TDZ+Adenine. Among the forty combinations tried, the high percent shoot regeneration was observed in peti-ole explants (76%) on MS medium supplemented with 0.22mg/l TDZ whereas in case of leaf ex-plants only 44% shoot regeneration was achieved on MS medium supplemented with 0.77mg/l TDZ+ 0.087 mg/l IAA. For root regener-ation the in vitro regenerated shoots were cul-tured on MS medium containing different con-centration of IAA and NAA. Hundred percent root regeneration was observed on the MS medi-um supplemented with 0.05mg/l and 0.20mg/l NAA respectively. The regenerated plantlets were transferred to the pots containing cocopeat and acclimatized. The regenerated plantlets were morphologically similar. An efficient and repro-ducible plant regeneration protocol has been standardized in cauliflower cv. Pusa Snowball K-1 and is being used for Agrobacterium- mediated cry1Aa gene transfer studies.

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Keywords

Cauliflower, Indirect organogenesis, TDZ, Shoot Regeneration, Brassica oleracea var. botrytis.

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