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Vegetos- An International Journal of Plant Research
Year : 2014, Volume : 27, Issue : 2
First page : ( 101) Last page : ( 111)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.
Article DOI : 10.5958/2229-4473.2014.00020.2

Agro-Morpho and Molecular Characterization of World Mini core Collections using Hypervariable Microsatellite Markers in Rice (Oryza sativa L.)

Soni Sandeep Kumar*, Yadav VK, Sundaram RM1, Balachandran SM1, Bhadana VP2, Virakatmath BC2, Pratap N3, Yadav MC4

Department of Biotechnology, Khalsa College, Amritsar, 143002, India

1Department of Genetics & Plant Breeding, CSA University of Agriculture & Technology, Kanpur-208 002 (U.P.) India

2Crop Improvement Section, DRR, Rajendranagar, Hyderabad-500 030 (A.P.) India

3Department of Genetics & Plant Breeding, ND University of Agriculture & Technology, Faizabad-224 229 (U.P.) India

4Division of Genomic Resources, NBPGR, Pusa Campus, New Delhi-110012, India

*Corresponding author E-mail: sandeepsoni71187@gmail.com

Online published on 20 October, 2014.

Abstract

Mini core collection plays an important role in evaluating genetic resources hence, an attempt was made to characterize world mini core collections on the basis of 35 uniformly distributed hyper variable SSR molecular markers. Thirty five markers amplified a total of 131 allelic fragments with an average of 3.28 alleles per locus indicating high level of polymorphism among the analyzed rice lines. The two major clusters shown by PPS SRS technique indicated no difference for grouping japonica and indica genotypes. Therefore the allele specific amplification pattern was adapted for identification of particular genotypes. The similarity coefficients among the genotypes ranged from 0.58 to 0.83 with an average similarity value of 0.36. Maximum similarity value of 88% was observed between PR33319-9-1-1-5-3-5-4-1 and PR-114. Whereas minimum similarity value of 54% was found between TJ 50836 and TJ 35724 followed by TJ 39050 & TJ 35724 and TJ 50836 and NDR359 genotypes with 55% of genetic similarity. Breeders worldwide could use these findings to choice the germplasm for transferring characters to local cultivars for grain yield, cooking quality and genetic classification.

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Keywords

Core collections, specific amplification pattern, descriptors, Genetic similarity, japonicaindica.

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