In vitro Improvement in Regeneration Potentiality by Using Kinetin Primed Seeds of Rice (var. MTU-7029)
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The present study deals with the somatic embryogenesis of rice (var. Swarna/MTU-7029), where a comparative characterization of the calli were made, using dehusked, sterilized whole seeds, obtained from kinetin primed and nonprimed one. For callus induction 30gL−1 sucrose + 1gL−1 casein hydrolysate and 30gL−1 maltose + 1gL−1 casein hydrolysate were added in MS medium separately which was supplemented with 2 mgL−1 2,4-D. In the regeneration medium (MSR1 to 6) different concentration of BAP, kinetin and NAA (2:0:1, 3:0:1, 4:0:1, 2:3:1, 3:2:1 and 4:1:1 mgL1) were used respectively. For regeneration of roots half strength MS basal medium was used supplemented with IBA 1mgL−1, sucrose 2% (w/v) and 0.8% (w/v) agar. Different concentrations (2.5 and 5 ppm) of the kinetin used for priming before using the seeds for inoculation in the culture medium, showed promising results regarding the total callus induction percentage, total embryogenic calli percentage, fresh and dry weights, proline content, nitrate reductase and superoxide dismutase activities in respect to non primed one. 2.5ppm (T2) kinetin primed seeds showed best result than 5ppm(T3) kinetin primed seeds. Among the entire regenerated medium, MSR6 showed highest regeneration (%), number of shoots regenerated per calli and recovery of plantlets from 50 tubes in all the treatments. The plantlets recovered from inoculated primed seeds calli have more number of roots in respect to non-primed one. Plantlets obtained from primed and non-primed control seeds were hardened in polyhouse and studied in detail upto the formation of panicle. On the basis of the above data T2 was found better than control.
Casein hydrolysate, Kinetin, Priming, Oryza sativa, Somatic embryogenesis.