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Research Journal of Science and Technology
Year : 2017, Volume : 9, Issue : 2
First page : ( 219) Last page : ( 223)
Print ISSN : 0975-4393. Online ISSN : 2349-2988.
Article DOI : 10.5958/2349-2988.2017.00038.9

Actinomycetes synthesized nanoparticles and their antibacterial activity

Thomas P. Danial*

Medical Laboratory Science, Dilla University and Referral Hospital, Ethiopia

*Corresponding Author E-mail: thomasbest@rediffmail.com

Online published on 18 September, 2017.

Abstract

One Gram negative bacterium (Bacillus subtilis) and two Gram positive bacteria (Pseudomonas aeruginosa and Escherichia coli) were tested against silver nanoparticles synthesized (Nps) by soil isolated actinomycetes for its antibacterial activities. The zone of inhibition of silver nanoparticles was measured. The results indicated that the silver nanoparticles synthesized from Actinomycetes showed effective antibacterial activity against all the test organisms. Among the three test organisms tested P. aeruginosa showed to be more susceptible (16 mm) against the Nps synthesized with a quantity of 40 μl followed by B.subtilis (11 mm) and E.coli (11 mm) with the same concentration of Nps. Previously the synthesized Nps were confirmed for their existence with the help of UV-Vis, SEM and FTIR. The actinomycetes synthesized Nps were found to be effective enough against pathogenic organisms and can be synthesized in a large scale which needed further research and analysis.

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Keywords

Actinomycetes, silver nitrate, nanoparticles, antibacterial.

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