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Research Journal of Science and Technology
Year : 2016, Volume : 8, Issue : 3
First page : ( 129) Last page : ( 134)
Print ISSN : 0975-4393. Online ISSN : 2349-2988.
Article DOI : 10.5958/2349-2988.2016.00017.6

Anti-microbial activity of Ethanol extracts obtained from different parts of Calotropis gigantea

Chand Tara1, Sharma Manoj Kr.2,*, Pandey Fanish Kr.3, Chakraborty Chaitali3

1National Institute of Biologicals, Noida

2Dabur Pvt. Ltd., Ghaziabad

3ITS, Paramedical College, Ghaziabad

*Corresponding Author E-mail: manojkr110@rediffmail.com

Online published on 18 October, 2016.

Abstract

The different extracts obtained from leaves, fruit, seeds, flower and stem of Calotropis gigantea were screened for its anti microbial and phytochemical activities. Ethanol and distilled water solvents were used for the extractions from these parts. The extract was tested against infectious diseases causing fungal pathogens such as Candida albicans and bacterial pathogens such as Staphylococcus aureus, Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa using the Agar well diffusion method. The Ethanol extract from the leaves of Calotropis gigantea showed more activity against fungus like Candida albicans zone of diameter 23.35 mm and bacteria like Staphylococcus aureus, Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa zone of diameters (in mm) were 22.50, 21.10, 30.08 and 19.70 respectively when compared with the extract obtained from other parts of C. gigantea such as fruit, seeds, flower and stem. In this study, both in bacteria and fungi Ethanol extract obtained from leaves showed a varying degree of inhibition of the growth of tested organism than extracts obtained from other parts of C. gigantea. The results confirmed that maximum presence of Antifungal and Antibacterial activity in the ethanol extract obtained from leaves of C. gigantea against the human pathogenic organisms.

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Keywords

Antimicrobial activity, ethanol extract, Leaves, stem, flower, seed and fruit.

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