(52.3.228.47)
Users online: 46    [ij] [ij] [ij] 
Email id
 

Research Journal of Science and Technology
Year : 2019, Volume : 11, Issue : 3
First page : ( 179) Last page : ( 182)
Print ISSN : 0975-4393. Online ISSN : 2349-2988.
Article DOI : 10.5958/2349-2988.2019.00027.5

Development and Validation of an RP-HPLC Method for Pamabrom in Bulk and Pharmaceutical Dosage Form

Jadhav P. B.*, Bhokare S. G., Madibone M. N.

Srinath College of Pharmacy, Aurangabad, Maharashtra, India

*Corresponding Author E-mail: sandhya.madala@gmail.com

Online published on 31 December, 2019.

Abstract

An accurate, Precise, Simple and Economical High Performance Liquid Chromatographic method for the estimation of Pamabrom in bulk form has been developed. The method so developed is Reverse Phase High Performance Liquid Chromatographic method using Primesil C18 column (Length: 250nm, Diameter: 4.6nm, Particle size: 5μ) with a simple methanol and Water (0.05% OPA) mixed in a proportion of 80: 20 v/v as mobile phase. The retention time for Pamabrom was found to be 4.48 min. The linearity for the method was observed in a concentration range of 10–50μg/mL with the correlation coefficient of 0.999. The method so developed was validated in compliance with the regulatory guidelines by using well developed Analytical method validation tool which comprises with the analytical method validation parameters like Linearity, Accuracy, Method precision, Specificity, System suitability, Robustness and Ruggedness. The results obtained were well within the acceptance criteria.

Top

Keywords

Pamabrom, RP-HPLC, Method development, Validation.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
445,161,253 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.