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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 9
First page : ( 1465) Last page : ( 1468)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00284.5

Detection of blaVIM gene encoding Metallo Beta Lactamase resistance among clinical isolates of Pseudomonas aeruginosa

Varshan R.1,*, Dr. Prakasam Gopinath2

1BDS 2nd year, Saveetha Dental College and Hospitals, Chennai, India

2Senior Lecturer, Department of Microbiology, Saveetha Dental College and Hospitals, Chennai, India

*Corresponding Author E-mail:

Online published on 12 January, 2017.

Abstract

In recent decades P. aeruginosa has emerged as a multidrug resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents. This bacteria uses distinctive resistant mechanisms to all the available antibiotics, which include metallo β-lactamases (MBL) production, extended spectrum β-lactamase production, AmpC production, decreased permeability, altered penicillin binding proteins and rarely, overexpression of efflux pumps. In this study we have taken 20 clinical isolates of P. aeruginosa and were subjected to antibiotic sensitivity pattern followed by the detection of blaVIM gene by PCR. Higher degree of resistance to routinely used antibiotics were reported. 85% of isolates were appeared to be positive for blaVIM gene by PCR. This indicates the important role of clinical microbiology laboratories to distinguish MBL-producing P. aeruginosa from strains with other mechanisms responsible for carbapenem resistance. The early detection of MBLproducing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multi drug resistant strains.

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Keywords

Pseudomonas aeruginosa, blaVIM gene, PCR, Metallo beta lactamase.

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