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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 4
First page : ( 394) Last page : ( 396)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00071.8

Assessing the Adhesion Properties of Isolated Probiotic Bacillus subtilis to Human Colonic Epithelial Cells

moorthy M. Krishna1,*, Nayak Bijaya Kumar2, Nanda Anima1, Anbarasi P.3

1Department of Biomedical Engineering, Sathyabama University, Chennai, Tamil Nadu, India, 600 119

2Department of Plant Science & Plant Biotechnology, KMCPGS, Airport Road, Lawspet, Pondicherry, India, 605 008

3Avanz Bio Private Limited, 12 MES Road, East Tambaram, Chennai, Tamil nadu, India, 600059

*Corresponding Author E-mail: krish2285@gmail.com

Online published on 6 June, 2016.

Abstract

Key criterion for selection of a Probiotic should have the adherence property of bacteria to human epithelial cells and mucosal surface. In this study we assess the adhesion property of selected indigenous probiotic Bacillus subtilis strain by the ability to adhere to human epithelial cells. (Yudhishtir, et, al. 2010) Bacillus subtilis is isolated from fermented plant beverage type coconut toddy, among 137 organisms Bacillus subtilis showed good probiotic characteristics further to its characterization it's studied for its adhesion property to human epithelial cells. Colonization potential is tested by using CaCo2 and HT29 colonic adenocarcinomal human intestinal epithelial cell lines. Bacillus subtilis strain that adhered to prescribed cell lines was also examined by plating in to the suitable medium by plating them after trypsinization. This colony shows that the isolated Bacillus subtilis has their ability to adhere to epithelial cells and thus this could have better prospects to colonize the gut with extended transit.

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Keywords

Adhesion, CaCo2, HT29, Bacillus subtilis, probiotic.

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