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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 10
First page : ( 1635) Last page : ( 1637)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00327.9

Detection of biofilm among clinical isolates of Acinetobacter baumannii by tissue culture plate method (TCP)

Bhavani G.1,*, Dr. P Gopinath2

1BDS 3rd year, Saveetha Dental College, Saveetha University, Chennai

2Senior Lecturer, Department of Microbiology, Saveetha Dental College, Saveetha University, Chennai

*Corresponding Author E-mail:

Online published on 2 March, 2017.


Acinetobacter baumannii can able to establish bio films on abiotic surfaces as well as biotic surfaces. The potential ability of A. baumannii to form bio films could explain its outstanding antibiotic resistance and survival properties in hospital environment. A sum of 20 clinical isolates of A. baumannii isolates were studied for antibiotic sensitivity pattern to routinely used antibiotics followed by the detection of biofilm using tissue culture plate method (TCP). We have observed increased percentage of resistance to all of the antibiotics tested. 70% of the isolates were biofilm formers. As biofilm formation, could resist antibiotics and making them stubborn pathogen in clinical setups. Thus, it is very important to study the biofilm by simple method like TCP for proper and accurate management of patient's outcome.



Acinetobacter Baumannii, Antibiotic Sensitivity Pattern, Tissue Culture Plate (TCP) Method, Biofilm.


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