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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 10
First page : ( 1626) Last page : ( 1628)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00324.3

Detection of CNA Gene for the presence of collagen adhesion in clinical strains of Staphylococcus aureus

Rupashri S.V.1, Dr. Gopinath P.2

1BDS 2nd year, Saveetha Dental College, Chennai

2Senior Lecturer, Department of Microbiology, Saveetha Dental College, Chennai

Corresponding Author E-mail:

Online published on 2 March, 2017.

Abstract

S. aureus has the ability to bind specific host matrix proteins. The S.aureus surface proteins (adhesins) that mediate the binding of these proteins iscalled as MSCRAMMs to denote their role as `microbial surface components recognizing adhesive matrix molecules ’. Genes encoding SaMSCRAMMs that bind collagen, fibronectin, fibrinogen, elastin and osteopontin have been identified. Unlike the genes encoding other S. aureus MSCRAMMs, the gene encoding the collagen-binding MSCRAMM (cna) is not present in every strain of S. aureus. In this study we have taken 20 clinical isolates of S. aureus and were subjected to antibiotic sensitivity pattern followed by the detection of cna gene by PCR. We have observed total resistance to penicillin followed by other group of drugs. 10% of our strains were found to have cna gene. As we had only limited isolates from restricted samples, it is important to include more number of isolates from especially bone related infections in order to rule out the same.

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Keywords

Staphylococcus aureus, CNA Gene, PCR Method.

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