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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 10
First page : ( 1623) Last page : ( 1625)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00323.1

Detection of blaTEM-1 gene for ESBL production among clinical isolates of Pseudomonas aeruginosa

Varshitha A1, Dr. Gopinath P2

1BDS 2 year, Saveetha Dental College, Saveetha University, Chennai

2Senior lecturer, Department of Microbiology, Saveetha University, Chennai

Corresponding Author E-mail:

Online published on 2 March, 2017.

Abstract

The occurrence of multi drug resistant P. aeruginosa strains is growing in the world, limiting the therapeutic options. Conventionally it was believed that ESBLs are derivatives of TEM and SHV parent enzymes but recently in Europe it was proved that ESBLs can be of non-TEM, non-SHV lineage. In this study we have taken 20 clinical isolates of P. aeruginosa and were subjected to antibiotic sensitivity pattern followed by the detection of blaTEM-1 gene by PCR. Higher degree of resistance to routinely used antibiotics is reported, 15% of them were found to have this gene by PCR. Proper detection of desired panel of genes is required to emphasize the resistance mechanism being operated in these pathogens for better outcome.

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Keywords

Pseudomonas aeruginosa, blaTEM-1, PCR, ESBLs.

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