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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 10
First page : ( 1618) Last page : ( 1620)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00321.8

Detection of blaNDM-1 gene for the production of MBL in clinical strains of Klebsiella pneumoniae

Reshmi B.1, Dr. Gopinath P.2

1BDS Student, Saveetha Dental College, Chennai

2Senior Lecturer, Department of Microbiology, Saveetha Dental College, Chennai

Corresponding Author E-mail:

Online published on 2 March, 2017.

Abstract

Spreading of NDM-1 producing K. pneumoniae in a clinical setting is a complex event involving several modes of spread, such as dissemination of several unrelated strains or the propagation of a single clone from patient to patient and from the environment to patients. A total of 20 clinical isolates of K. pneumoniae were subjected to antibiotic susceptibility pattern followed by the detection of blaNDM-1gene by PCR. Our results indicates the increased degree of resistance to most of the routinely used antibiotics. 10% of our isolates were found to posses this gene among our isolates. Early detection of these genes will help in prevention and adequate infection control by limiting the spread of these organisms.

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Keywords

Klebsiella pneumoniae, blaNDM-1, Carbapenems, PCR.

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