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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 4
First page : ( 462) Last page : ( 467)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00077.3

Isolation of Flavonoids and their Anticancer Activity from Sphaeranthus amaranthoides in A549 Cell Line

Swarnalatha Y.*

Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Chennai-119

*Corresponding Author E-mail: lokiswarna@gmail.com

Abstract

Flavonoids are considered as potential explore to treat cancer and they are ubiquitous. The chemical structure and the nature of the polyphenolic flavonoids are responsible for their anticancer actions. Objective: The present investigation concentrates on the cytotoxic effect of flavonoids in A549 adenocarcinoma cell lines. Methodology: the plant extract was subjected to TLC for confirmation and HPLC confirm the pure form of the flavonoids and the induction of the cytotoxicity was studied using AO/EB, PI and the uptake of the PI was confirmed using the FACS in A549 cells. results: the TLC and HPLC results confirmed the presence of flavonoides. Flow cytometry denotes the percentage of the cells in the G0/G1 and the G2 stage. This indicates the accumulation of the cells in the apoptotic and proapoptotic stages. Conclusion: These findings indicate that the flavonoids from the Sphaeranthus amarathoides may be promising anticancer agents to control the cell growth in A549 cells.

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Keywords

Flavonoids, cancer, FACS, A549, Sphaeranthus amaranthoides.

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