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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 4
First page : ( 349) Last page : ( 354)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00058.X

Optimization of Cellulase Producing Aspergillus flavus SB4 by Solid State Fermentation using Response Surface Methodology (RSM)-CCD

Utharalakshmi N.1,*, Kumar A. Ganesh2, Narendrakumar G.1

1Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai-600119, India

2National Institute of Ocean Technology, NIOT Campus, Chennai -600 100, India

*Corresponding Author E-mail: uthiral@gmail.com

Abstract

Solid State fermentation is widely applied to produce many enzymes and metabolites mainly from fungi. Multiple substrate (Paddy straw, Sugarcane bagasse, Rice husk and wheat bran) were mixed in different combination. Aspergillus flavus isolated from rhizosphere was inoculated. After incubation, the cellulase concentration was quantified using FPase. The optimum parameter for the production of cellulase was analysed using RSM –CCD. The results were evaluated on 28th day of incubation. R2 value was 0.989, adjusted R2 was 0.9788 and predicted R2 is 0.9505. The quadratic model gave a satisfactory description of the experimental data. Under optimum conditions, a maximum cellulase concentration of 55.43 IU/g was predicted according to the developed model.

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Keywords

Aspergillus flavus, SSF, Optimization, RSM-CCD, cellulase.

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