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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 10
First page : ( 1438) Last page : ( 1444)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00258.9

In-Vitro and in-Vivo Anti-Inflammatory Activities of Salvia hispanica and Linum usitatissium Seeds in Swiss Albino Rats

Vidyasabbani1,*, Ramesh A.1, Snehalatha2, Rahul Bhanu2, Sriharitha2, Sanjayvarma2, Aparna2

1Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Medak, Telangana, India

2Department of Pharmacology, Sitha Institute of Pharmaceutical Sciences, Rajivgandhi Nagar, Hyderabad, Telangana, India

*Corresponding Author E-mail: vidya.s@viper.ac.in

Online published on 2 December, 2015.

Abstract

Salvia hispanica called as Chia seeds family Lamiaceae and Linum usitatissium called as Flax seeds family Linaceae was evaluated for its invitro and invivo anti-anti inflammatory activities individually and combined in swiss albio rats. Phytochemical screening analysis of both seeds revealed the presence of flavanoids. The Fourier Transform Infrared Spectrophotometer (FTIR) analysis of chia and flax seeds confirmed the presence of amide, alcohols, phenols, alkanes, carboxylic acids, aldehydes, ketones, alkenes, primary amines, aromatics, esters, ethers, alkyl halides and aliphatic amines compounds, which showed major peaks. In-vivo anti-inflammatory activity was done on Swiss albino rats (150–250 gms) at two different doses (100 and 200 mg/kg body weight) by using 1% histamine induced paw edema test. In-vitro anti-inflammatory test was done by proteindenaturation and the human red blood cell (HRBC) membrane stabilization method. Data were analyzed by oneway analysis of variance (ANOVA) followed by Dunnett's t-test and the test of significance P value <0.05 was considered as the minimum level of significance. The paw volumes and percentages of inhibition of inflammation with chia and flax and standard drugs are reported and were observed that the seeds of chia and flax combination showed Synergistic action of inhibition at two doses compared to individual seeds. The inhibition was the highest at 3 h at 200 mg/kg dose which was slightly lower than Indomethacin effect. In the study of membrane stabilization activity of chia and flax combination at concentration range of 100–200 μg/ml protected significantly in a concentration dependent manner the erythrocyte membrane against lysis induced by hypotonic solution. Acetylsalicylic acid in the concentration of 100 μg/ml used as standard also offered significant (p<0.001) protection of RBC's membrane against damaging effect induced by hypotonic solution. The present research concludes that seeds of Salvia hispanica and Linum usitatissium possess anti-inflammatory activity in dose dependant manner and also proved synergistic activity.

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Keywords

Anti-inflammatory, Salvia hispanica and Linum usitatissium, Protein-denaturation, Indomethacin, Phytochemical screening, Human red blood cell (HRBC), Membrane stabilization.

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