Chromatographic Finger Print analysis of Dryopteris cochleata by HPTLC technique
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To evaluate the phytoconstituents composition and HPTLC fingerprint sequence profile of the medicinally important plant dried rhizome of Dryopteris cochleata (Dryopteridaceae).
Preliminary phytochemical screening was done by the method as described Kokate. The HPTLC fingerprint analyses were carried out in two different solvent systems, which showed different Rf value as Harborne and Wagner et al described. HPTLC analysis was done using CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software. The Chloroform: Methanol (9: 1) and Toluene: Methnol (9: 1) were employed as mobile phase.
The phytochemical screening showed the presence of various phytocompounds. The HPTLC fingerprinting of the rhizome extracts showed several peaks with different Rf values. The ethanol extract revealed 6 peaks in Chloroform: Methanol (9: 1) mobile phase whereas 4 peaks in Toluene: Methanol (9: 1) mobile phase.
It can be concluded that different Rf value of various phytoconstituents provide valuable clue regarding their polarity and selection of solvents for separation of active phytoconstituents. Such finger printing is useful in differentiating the species from adulterants and act as a biochemical marker for develop the standardization parameters of the plant.
Phytochemical screening, HPTLC finger printing, Dryopteris cochleata.