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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 10
First page : ( 1394) Last page : ( 1398)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00250.4

Chromatographic Finger Print analysis of Dryopteris cochleata by HPTLC technique

Das Sattwik1,*, Lariya Shailendra2, Mistry Sunil3

1Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, India

2Radha raman Institute of Pharmaceutical Science, Bhopal, Madhya Pradesh, India

3Malhotra College of Pharmacy, Bhopal, Madhya Pradesh, India

*Corresponding Author E-mail: dsattwik@gmail.com

Online published on 2 December, 2015.

Abstract

Objective

To evaluate the phytoconstituents composition and HPTLC fingerprint sequence profile of the medicinally important plant dried rhizome of Dryopteris cochleata (Dryopteridaceae).

Methods

Preliminary phytochemical screening was done by the method as described Kokate. The HPTLC fingerprint analyses were carried out in two different solvent systems, which showed different Rf value as Harborne and Wagner et al described. HPTLC analysis was done using CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software. The Chloroform: Methanol (9: 1) and Toluene: Methnol (9: 1) were employed as mobile phase.

Results

The phytochemical screening showed the presence of various phytocompounds. The HPTLC fingerprinting of the rhizome extracts showed several peaks with different Rf values. The ethanol extract revealed 6 peaks in Chloroform: Methanol (9: 1) mobile phase whereas 4 peaks in Toluene: Methanol (9: 1) mobile phase.

Conclusions

It can be concluded that different Rf value of various phytoconstituents provide valuable clue regarding their polarity and selection of solvents for separation of active phytoconstituents. Such finger printing is useful in differentiating the species from adulterants and act as a biochemical marker for develop the standardization parameters of the plant.

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Keywords

Phytochemical screening, HPTLC finger printing, Dryopteris cochleata.

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