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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 10
First page : ( 1379) Last page : ( 1382)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00247.4

Isolation and Purification of Lutein from Indian Spinach Basella alba

Prabhu Ashwini1, Abdul Kadar Sajida2, Rekha Punchappady-Devasya1,*

1Yenepoya Research Centre, Yenepoya University, Mangalore-575 018, Karnataka, India

2Department of Postgraduate Studies and Research in Biosciences, Mangalore University, Mangalagangotri-574 199, Karnataka, India

*Corresponding Author E-mail: rekhapd@hotmail.com

Online published on 2 December, 2015.

Abstract

Lutein is a pharmacologically important phytochemical belonging to the class of carotenoids widely distributed in marigold flowers and green vegetables. It is industrially important for its biological activities such as antioxidant, immunostimulatory and macular protective properties. In this study, isolation and purification of lutein from Indian spinach, Basella alba was carried out. For the extraction of lutein, petroleum ether-acetone extract of spinach leaves was prepared which yielded 2.3 mg/100g of lutein. Preparative TLC of the extract yielded lutein with a Rf value of 0.12. Lutein isolated was subjected to spectral characterization by UV-Visible and FT-IR spectroscopy. The peaks obtained corresponded well with those reported for standard lutein. Results of the study indicated that B. alba leaves can be used as a source for obtaining lutein.

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Keywords

Basella alba, Lutein, Solvent extraction, Spectral characterization.

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