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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 1
First page : ( 1) Last page : ( 5)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00001.3

Analytical Method Development and Validation for Simultaneous Estimation of Montelukast and Ebastine by HPLC

Shrikrishna Baokar1,2,*, Nisharani Ranpise3

1Shivnagar Vidya Prasarak Mandal's College of Pharmacy, Malegaon (Bk.), Tal-Baramati, Dist-Pune, Maharashtra, India-413115

2Department of Pharmaceutical Sciences, Pacific Academy of Higher Education and Research (PAHER) University, Udaipur, Rajasthan, India-313003

3Department of Pharmaceutical Sciences, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune, Off Sinhgad Road, Pune, Maharashtra, India-411041

*Corresponding Author E-mail: krishnabaokar@gmail.com

Abstract

A RP-HPLC method was developed and validated for simultaneous estimation of montelukast and ebastine in bulk as well as in tablet formulation according to ICH guidelines. The chromatographic separations of drugs were achieved on Younglin (S.K) isocratic system with Lichrocart C18 column (4.6 mm×250 mm, 5μm). The mobile phase consisted by methanol and water (80: 20) at pH 3 adjusted by ortho phosphoric acid. The flow rate was adjusted to 1 ml/min and UV detection was carried out at 268 nm. The retention time for montelukast and ebastine were found to be 6.56 and 2.95 min respectively. The detector was showed linear responses over the concentration range 5 – 25 μg/mL for both drugs by showing a good correlation coefficient of 0.999. This proposed method is highly sensitive, precise and accurate which reduces cost of analysis; hence recommended for routine quality analysis in laboratories.

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Keywords

Montelukast, ebastine, validation, simultaneous estimation.

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