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Research Journal of Pharmacy and Technology
Year : 2014, Volume : 7, Issue : 7
First page : ( 749) Last page : ( 753)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.

Development and Validation of Analytical Method for Simultaneous Estimation of Curcumin and Gallic Acid in Different Polyherbal Formulations by HPLC

Purohit Pratik J.*, Kapupara Pankaj P., Shah Ketan V.

Department of Quality assurance, School of Pharmacy, RK University, Kasturbadham, Rajkot-360 020

*Corresponding Author E-mail: jayparasuram@gmail.com

Online published on 29 August, 2014.


A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of Curcumin and Gallic acid from polyherbal dosage forms. The method was carried out on a Monolithic (C18 (10 X 4.6 mm, 5 μm)) column with a mobile phase consisting of methanol: 0.1% triflouroacetic acid at a flow rate of 1.0 ml/min. Detection was carried out at 296 nm. The retention time of Curcumin and Gallic acid was 3.91 and 1.90 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation. The proposed method can be used for the estimation of these drugs in combined polyherbal dosage forms. The standardization of capsule formulations was performed by determining loss on drying, total ash, acid insoluble ash, water soluble ash, alcohol soluble extractive value, water soluble extractive value etc. according to WHO guidelines.



Herbal Drugs, Curcumin, Gallic acid, Simultaneous method, Method validation, Standardization, Marketed formulation.


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