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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 9
First page : ( 4334) Last page : ( 4338)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00766.0

A new rp-hplc method development and validation of paracetamol and aceclofenac in tablets by separating diclofenac

Chandrasekhar Kudupudi*, Manikandan A.

Department of Science and Humanities, Bharath Institution of Higher Education and Research, Selaiyur, Chennai, Tamilnadu-600073, India

*Corresponding Author E-mail: kudupudichandra@gmail.com

Online published on 16 September, 2020.


A new simple precise, accurate and selective RP-HPLC method was developed for the simultaneous estimation of Paracetamol and Aceclofenac in their mixed formulation by separating Diclofenac. All the three drugs were separated on Kromasil C18 (150x4.6, 5μ) with reverse phase elution of the mobile phase compose of 0.05M potassium dihydrogen phosphate and Acetonitrile in the ratio 40:60 v/v at a flow rate of 1.0mL/min. The detection was made at 275nm. The retention times were 2.36 min for Paracetamol, 3.23 min for Aceclofeanc and 6.38 min for Diclofenac. The linearity ranges for paracetamol and aceclofenac were 16.25 to 48.75 and 5 to 15 mcg/ml respectively with correlation coefficients 0.999 and 1.0. The proposed method statistically validated with respect to system suitability, specificity, linearity, range, precision, accuracy, robustness and ruggedness. The method was accurate, linear, precise, specific, selective and rapid found to be suitable for the quantitative estimation of Paracetamol and Aceclofenac by separating Diclofenac.



Stability-indicating, Paracetamol, Aceclofenac, Diclofenac, Tablets, RP-HPLC.


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