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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 9
First page : ( 4111) Last page : ( 4114)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00726.X

Optimization of xylanase production from penicillium funiculosum using agricultural (Corn cob) waste

Burugu Abishna, Addanki Mounika, Surepalli Sobhitha, Chanda Chandrasekhar*

Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India-522502

*Corresponding Author E-mail: chandrasekharchanda02@gmail.com

Online published on 16 September, 2020.

Abstract

Xylanases are the recent breakthroughs in food, feed and pharma industries. Extensive research on xylanases will be beneficial to minimize the economy and high yield to the increased demands of humankind. Xylanases reduce plant cell structural polysaccharide called xylan to its subsequent sugar, xylose. Current study focused on the production and purification of xylanases from a fungal strain penicillium funiculosum taking agricultural waste i.e., corn cob powder as the major carbon source through solid state fermentation. Cation exchanger chromatography (CM Sephadex C-50) was used for fractionation of proteins and purification of xylanases. Approximately 668 Units of xylanase enzymeper gram of Corn Cob powder was produced in solid state fermentation.

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Keywords

Penicillium funiculosum, Xylanase, Xylan, Corn cob powder, Column chromatography.

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