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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 8
First page : ( 3861) Last page : ( 3866)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00683.6

Genetic fingerprinting of herbal medicinal plant-Lannea corromandelica linn. using pcr-rflp

Kumar Tekeshwar1, Vyas Amber2, Jain Vishal2,*

1M J College, Kohka, Junwani Road, Bhilai, Durg, Chhattisgarh, 490 023, India

2University Institute of Pharmacy, Pt. Ravishankar University, Raipur, Chhattisgarh, 492 010, India

*Corresponding Author E-mail: vish_106@rediffmail.com

Online published on 16 September, 2020.

Abstract

DNA based authentication techniques have been extensively used as a method for herbal drugs in present days. Consumption of herbal drugs is assumed as a safe, but there are some reported serious reaction demands development of effectual markers system for isolation and identification of the phyto-constituents. Numerous analytical and molecular techniques have also been developed for quality control of drugs from plant origin, but still there is no universal consensus to adopt the same. In this approach, we set to solve this problem by DNA based technique by developing a universal primer PCR-RFLP assay by developing genetic marker. These method use different variable lengths of mitochondrial DNA for amplification using a pair of universal primers. PCR amplifications yielded 531 bp length fragments of L. coromandelica. The limit of detection in range of 0.01 to 0.05 ng of genomic DNA. This developed PCR-RFLP assay was sufficient to distinguish with variable fragment patterns.

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Keywords

PCR, Restriction Enzyme, L. coromandelica, RFLP, Genetic fingerprint.

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