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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 8
First page : ( 3725) Last page : ( 3728)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00659.9

Bio-analytical method development and validation of dichlorvos pesticide by rp-uflc method

Lal C. L Vaishnav1, Chandan R. S2,*, Maruthi R, Tengli Anand Kumar

1Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru

2JSS Academy of Higher Education and Research, Sri Shivarathreeshwara Nagara, Mysuru, Karnataka, India, 570015

*Corresponding Author E-mail: rschandan@jssuni.edu.in

Online published on 16 September, 2020.

Abstract

Organophosphates are a group of pesticides that were developed in the 1940s in Germany andsoon became a significant defense against agricultural pests. The organophosphate Dichlorvos is a commonly used group of pesticide is a broad-spectrum organophosphate compound having insecticidal activity. Dichlorvos is a cholinesterase inhibitor exhibiting stomach, contact and systemic mode of action. The development of bioanalytical method is the process of developing a procedure for the detection and quantification of a compound of interest in a biological fluid. A simple, reliable, fast, accurate and economical RP-UFLC method have been developed and validated for quantitative determination of Dichlorvos. The RP-UFLC procedure is developed by using ACN and Millipore water 50:50 v/v as mobile phase, Flow rate is maintained at 1.5mL/minute. Detection of Dichlorvos was performed by using PDA detector at 200nm. By this RP-UFLC procedure retention time (RT) of Dichlorvos was identified at 2.9 min.

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Keywords

PDA detector, RP-UFLC method, Dichlorvos, Organophosphate, Bio-analytical.

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