Association between Trichomonas vaginalis and vaginal bacterial community composition in Human vagina
*Corresponding Author E-mail: Hudaalmarsome@yahoo.com
Trichomonas vaginalis is a parasite that is seen inside the vagina of the human body. Normally the vaginal PH is acidic. The vaginal PH should be changed to alkaline for opposing the infection of the disease. From the previous studies, it's clear that the vaginal microbiota of a mature woman can be divided into five kinds of bacteria. Four of these types are dominated by Lactobacillus bacteria. But, one among them is dominated by bacteria other than Lactobacillus.
Aim of the study
Is to find the relationship between Trichomonas vaginalis and bacteria collected from the vagina of Iraqi women.
Patients and Methods
from the Gynecology Department of Al Imamein Al Kadhimein Medical in Iraq, we collected vaginal samples from 100 Iraqi women. This was done during the period between May 2014 and April 2015. Females with discharge and alkaline PH enrolled in the study. Trichomonas vaginalis is detected mainly by two methods. A polymerase chain reaction can be conducted and vaginal microbiota can be examined by the analysis of 16 sRNA genes. Samples were sent to Macrogen/Korea.
From hundred vaginal swabs only sixty were alkaline. 12 were positive for the parasite by PCR. Five samples were sequence for 16 RNA. Uncultured Lactobacillus were detected in four samples., So the 4CST are common in this study, one sample represent CST-IV, Sample 5 were common with Sterptophyta. uncultured method like NGs is helpful for diagnosis of uncultured bacteria. Lactobacillus spp.(uncultured) were dominated in our samples which might play a role in established parasite infection.
Trichomonas vaginalis, vaginal Bacteria, human.