(34.204.187.106)
Users online: 2694    [ij] [ij] [ij] 
Email id
 

Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 6
First page : ( 2815) Last page : ( 2818)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00501.6

Phytochemical and pharmacological screening of leaf of Lannea coromandelica Linn.

Kumar Tekeshwar1, Vyas Amber2, Jain Vishal2,*

1M J College, Kohka, Junwani Road, Bhilai, Durg, Chhattisgarh, 491 023, India

2University Institute of Pharmacy, Pt. Ravishankar University, Raipur, Chhattisgarh, 492 010, India

*Corresponding Author E-mail: vish_106@rediffmail.com, ambervyas@gmail.com

Online published on 4 July, 2020.

Abstract

WHO is highly recommended the customary exploitation of traditional therapeutics of medicinal plants and is consequently in pipeline for development of monograph and exploring their uncultivated pharmacological properties. Presently, correct identification of medicinal plants by phytochemical fingerprints is a speedy tool to ensure reproducible quality of herbal drugs. In our study, the ethyl acetate and methanol extract of leaves of Lannea coromandelica (L. coromandelica) was subjected for chemical fingerprint as well therapeutic aid as antifilarial agents. High performance thin layer chromatography (HPTLC) was employed to develop chemical fingerprint and filariciadal activity was assessed by motility inhibition and MTT reduction assay with concentrations range 1000 to 25μg/ml. The HPTLC analysis of ethyl acetate and methanol extract was carried out using hexane: ethyl acetate: formic acid: methanol (5:4:1:0.5 v/v/v/v) for fingerprinting and quantification ellagic acid and quercetin. The HPTLC method was found to give compact spots for Rf = 0.54 and 0.51 ellagic acid; Rf = 0.91 and 0.87 quercetin for ethyl acetate and methanol extract respectively. The HPTLC method was validated as per the ICH guidelines. Inhibitory concentration (IC50) for the ethyl acetate extract was found to be 284.5μg/ml. In motility assay, complete inhibition of motility was observed for all concentrations. Hence, our study could be valuable for inventing strategies for quality control parameter and justifies the ethnic uses of plant in folkloric medicines.

Top

Keywords

WHO, Lannea coromandelica, HPTLC, filariciadal activity.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
429,689,130 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.