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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 6
First page : ( 2624) Last page : ( 2628)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00466.7

In Vitro Antioxidant and Antibacterial Screening of flowers Extract from Cassia auriculata Linn.

Bargah Rohit Kumar*, Kushwaha Anil, Tirkey Arun, Hariwanshi Bimlesh

Department of Chemistry, Govt. S.P.M. College, Sitapur, Surguja (C.G.) India, 497111

*Corresponding Author E-mail: rohitbargah1978@gmail.com

Online published on 4 July, 2020.

Abstract

Nature has been a powerful source of enormous medicines for thousands of years and number of modern drugs has been extracted and exploited from natural sources, which are used in traditional systems of medicines. The main aim of the present study was to evaluate. In-Vitro antioxidant, antibacterial activity and preliminary phytochemical screening of the ethanolic and aqueous extract of flowers of Cassia auriculata Linn. Belonging to the family Caesalpiniaceae. Phytochemicals screening of the crude extract revealed the presence of flavonoids, steroids, saponins, phenolic compound, terpenoids, carbohydrates, were present in all extract while alkaloids and tannins were absent only in ethanolic extract. The antioxidant activity of flowers of Cassia auriculata was assessed with the help of antioxidant activities like 2, 2-Diphenyl-1-picryl hydrazyl (DPPH) scavenging assay. Total phenol and flavonoids content were measured. Flowers show most antioxidant activity in both extracts due to higher phenolic and flavonoids content that indicated the direct correlation among antioxidant potential, total phenol and flavonoids. The presence of the bioactive constituents have been linked to the antimicrobial activity of the plant materials against gram positive and gram negative bacteria using agar disc diffusion method. Minimum inhibition concentration value ranged from 50, 75, 100 μg/ml. Ethanolic extract exhibited significantly good antibacterial activity in dose depended manner. Therefore, it is concluded that Cassia auriculata possess phenolic properties that has considerable antioxidant and antibacterial properties.

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Keywords

Cassia auriculata, Antioxidant activity, Antibacterial activity, Phytochemical Screening, ethanolic extract, Flavonoids.

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