The Effect of Eleutherine americana Merr. Extract on Expression Changes of MMP-8 and Type 1 Collagen in Periodontitis Rat Models Yani Sinar1,2, Soeharto Setyawati1,3, Sumarno1,4, Kalim Handono1,5 1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya 2Oral Biology Laboratory, Faculty of Medicine, Universitas Mulawarman 3Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya 4Department of Microbiology, Faculty of Medicine, Universitas Brawijaya 5Rheumatology and Allergy-Immunology Division Internal Medicine, Department, Faculty of Medicine, Universitas Brawijaya *Corresponding Author E-mail: Sinaryani@yahoo.com
Online published on 16 June, 2020. Abstract Background Matrix Metalloproteinase (MMP-8) and type 1 collagen play an important role in the pathogenesis of periodontitis. This allows it to be used as a biomarker for evaluating the therapeutic effect. Various studies have shown that active components possessed Eleutherine americana Merr (EAM) has anti-inflammatory effects. The purpose of this study was to determine the expression change of MMP-8 as well as type 1 collagen after treatment with EAM in rats with periodontitis. Material and Methods Thirty-six rats (Rattus novergicus) were used as periodontitis rat models. Wire ligature was placed in a subgingival position, encircling entire cervix of the incisors. Periodontitis bacteria Porphyromonas gingivalis, was then injected into gingival sulcus twice a week for a maximum of 14 days. After the ligature placement, animals were randomly assigned to six experimental groups (n=6): 1) Periodontitis + Na-CMC, 2) Periodontitis + EAM100mg/Kg, 3) Periodontitis + EAM250mg/Kg, 4) Periodontitis + EAM500mg/Kg, 5) Periodontitis + EAM1000mg/Kg, 6) Periodontitis + Doxycycline. The experiment was conducted for 10 days and at the end of experiment, all the rats were sacrificed and their gingival tissue was removed. Expression of MMP-8 and type 1 collagen in gingival tissue was observed immunohistochemically. The data was then analyzed using a one-way ANOVA test. Results The gingiva of rats showed a significant decrease in MMP-8 expression after treatment with EAM250, EAM500 and EAM1000. Type 1 Collagen expression in gingiva increased significantly after treatment with ethanol extract of Eleutherine americana Merr in accordance with the dose. Conclusion Eleutherine americana Merr decreases expression of Matrix-Metalloproteinase 8 (MMP-8) and increased expression of type 1 collagen in gingival tissue of the periodontitis rat models. Top Keywords Eleutherine, MMP-8, Type 1 Collagen, Periodontitis, Rat. Top |