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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 5
First page : ( 2238) Last page : ( 2240)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00402.3

Eae A gene Detection in E. coli from Toddler diarrhea cases in Center of Babylon province

Aljarah Ali Kadhim1,*, Saad Ali Malik2, Kadhim Raad A.2

1Department of Microbiology, College of Veterinary Medicine, University of Kerbala, Iraq

2Department of Biology, Collage of Science for Women, University of Babylon, Iraq

*Corresponding Author E-mail: alialjarrah78@gmail.com

Online published on 16 June, 2020.

Abstract

Background

E. coli is considered as one of most important normal flora in animal and human gut. It has many serotypes and it could be isolated from feces of healthy individuals and patients. The aim of current study was the molecular genetics detection of presence of the amplified eae A gene fragment (384bp) to confirm presence of the E. coli.

Methods

A total of 36 feces samples were collected from patent with diarrhea at Mirjan Medical city from February 2016 till April 2016. Samples were cultured, identified and followed by DNA extraction. Using PCR amplification for diagnosis of E. coli bacteria in feces of toddlers with diarrhea by notification of eae A gene fragment (384bp).

Results

This study showed that eae A gene was found in 26 out of 32 cases with a rate of infection reaching 81.3%.

Conclusion

Eae A gene is responsible for the virulence in E. coli pathogenic bacteria.

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Keywords

Eae A gene, E. coli, Toddler diarrhea, PCR, test sensitivity.

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