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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 5
First page : ( 2233) Last page : ( 2237)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00401.1

Validated UV Spectroscopic Method for the Quantitative Determination of Ubidecarenone

Anandakumar K.1,*, Sangeetha V. P.1, Sindhuja V.1, Iswarya S. Sree1, Ramesh J.1, Arun N T.1, Raja M.2

1Department of Pharmaceutical Analysis, Swamy Vivekanandha College of Pharmacy, Elayampalayam, Tiruchengode-637205, Namakkal (DT), Tamil Nadu

2Department of Pharmaceutics, JSS College of Pharmacy, Ooty-643001, Tamil Nadu

*Corresponding Author E-mail: anandanalysis@gmail.com

Online published on 16 June, 2020.

Abstract

A new, simple, precise, accurate, sensitive and reproducible UV spectroscopic method for the estimation of Ubidecarenone in bulk and in capsule formulation was developed and validated. Shimadzu AUX-220 Digital balance was used for weighing purpose, Perkin Elmer Double beam UV Visible Spectrophotometer with pair of 10mm matched quartz cells, pre-aligned Deuterium and halogen lamp as a light source and photodiode detector was used for the analysis. Ethanol was used as a solvent. The λmax was found to be 275nm. The drug obeyed Beer's law in the concentration range of 10–80μg/ml with correlation coefficient (r2) of 0.9996. The LOD and LOQ for the developed UV Spectroscopic method were found to be 0.0371μg/ml and 0.1125μg/ml, respectively. The amount of Ubidecarenone in the capsule formulation was found to be 98.78±1.3682. The percentage recovery was found to be in the range of 98.95–100.10 for Ubidecarenone. The % RSD was found to be 0.5190 ± 0.1722. The % RSD value for the intraday and inter day analysis of Ubidecarenone was found to be 0.3921 and 0.5268, respectively. The % RSD was found to be less than 2. The developed method was validated as per ICH guidelines. The percentage recovery obtained indicated that there is no interference due to the excipients used in the formulation. Hence the developed method was found to be accurate. The proposed method can be successfully applied for the routine quality control analysis of Ubidecarenone in bulk and in capsule formulation.

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Keywords

Ubidecarenone, Ethanol, UV Spectroscopy, Validation, ICH guidelines.

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