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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 5
First page : ( 2087) Last page : ( 2090)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00375.3

Evaluation of Quantitative and GC-MS Analysis of Bioactive Compounds in Aqueous and Ethanolic extracts of Apium leptophyllum Pers

Ganesh M. Siva1,*, Radhika J.2

1Research Scholar, Department of Biochemistry, Srimad Andavan Arts and Science College, (Autonomous) Affiliated to Bharathidasan University, Trichy-620005, Tamil Nadu, India

2Research Supervisor, Department of Biochemistry, Srimad Andavan Arts and Science College, (Autonomous) Affiliated to Bharathidasan University, Trichy-620005, Tamil Nadu, India

*Corresponding Author E-mail: msgbiochem17@gmail.com

Online published on 16 June, 2020.

Abstract

Aim

The aim of the present work was to evaluate the quantitative estimation of secondary metabolites such as phenol and flavonoids in different extracts of Apium leptophyllum Pers and identification of bioactive compounds by GC-MS analysis.

Methods

Determination of phenol and flavonoids in aqueous and ethanolic extracts of Apium leptophyllum Pers by using UV-Visible spectrophotometric quantitative method and the ethanolic extracts of plant active compounds were identified by GC-MS analysis.

Results

The total phenolic content in aqueous and ethanol extract of Apium leptophyllum Pers were found to be 57.7±0.32 mg and 60.3± 0.87 mg of Gallic acid equivalents/100g of dry mass respectively, whereas the total flavonoid content of aqueous and ethanol extract was found to be 27.4± 0.76 mg and 39.8± 0.91mg Quercetin equivalents/100 g of dry mass. Thymol compound was highly found in the ethanolic extract of the plant. It was the richest source of ethanolic extract reflected in highest peak in GC-MS Chromatogram based on the percentage of peak area and compounds were identified based on Retention Time (RT Value)

Conclusion

The ethanolic extract has high content of phenol and flavonoids when compared to aqueous extract of Apium leptophyllum Pers and also evaluated the essential bioactive compounds have found in ethanolic extract of the plant by GC-MS analysis.

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Keywords

Apium leptophyllum pers, Aqueous and Ethanolic extracts, Quantitative method and GC-MS analysis.

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