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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 4
First page : ( 1883) Last page : ( 1887)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00339.X

Antimicrobial activity of Phyllantus niruri extract on Avian pathogenic Escherichia coli Isolated from Chicken with Colibacillosis symptoms

Sabdoningrum Emy Koestanti1,*, Hidanah Sri1, Yuniarti Wiwik Misaco2, Chusniati Sri3, Warsito Sunaryo Hadi1,*, Muchtaromah Bayyinatul4

1Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Jalan Mulyorejo, Surabaya, East Java, Indonesia, 60115

2Department of Clinical Science, Faculty of Veterinary Medicine, Airlangga University, Jalan Mulyorejo, Surabaya, East Java, Indonesia, 60115

3Department of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Jalan Mulyorejo, Surabaya, East Java, Indonesia, 60115

4Department of Biology, Faculty of Science and Technology, Maulana Malik Ibrahim State Islamic University Malang, Jalan Mulyorejo, Surabaya, East Java, Indonesia, 60115

*Corresponding Author E-mail: emykoestanti@yahoo.co.id

Online published on 30 April, 2020.

Abstract

Objective

In this study, we evaluated the antimicrobial properties of methanol extracts of Phyllantus niruri on avian pathogenic Escherichia coli associated with avian colibacillosis isolated from areas of East Java, Indonesia. We compared the activity of the extracts with oxytetracycline, gentamycin, enrofloxacin and ciprofloxacin.

Materials and Methods

This research used 60 samples taken from the infundibulum of layer chickens from 10 districts in East Java, Indonesia (Sidoarjo, Mojokerto, Kediri, Blitar, Bojonegoro, Jombang, Lamongan, Tuban, Jember and Banyuwangi) with 6 samples from each district.

Results

The results showed that 6 samples collected from Sidoarjo, Blitar, Bojonegoro, Jombang, Tuban, and Jember were E. coli positive and 4 samples from Lamongan, Mojokerto, Kediri, and Banyuwangi were negative. Polymerase Chain Reaction showed that seven isolates of E. coli were positive for the presence of the yaiO gene using a 115 base pairs amplified fragment. An MBC test showed that 25 and 50 mg/ml concentrations of P. niruri extract were lethal against E. coli. Ciprofloxacin also affected E. coli, but these strains were resistant to oxytetracycline, gentamycin and enrofloxacin at the same dose.

Conclusion

This study indicated that the P. niruri extract has antibacterial activity and has the potential to be used as a source for a new broad-spectrum oral antibiotic.

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Keywords

Antimicrobial activity, Escherichia coli, Minimum Bactericidal Concentration, Minimum Inhibitory Concentration, Phyllantus niruri extract.

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