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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 4
First page : ( 1774) Last page : ( 1778)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00320.0

Method Development and Validation for Estimation of Teneligliptin in Tablet Dosage Form by RP-HPLC

Biswas Bhanu1, Kumar Manish1, Sharma Jai Bharti1, Saini Vipin2, Bhatt Shailendra1,*

1M.M. College of Pharmacy, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala

2Maharishi Markandeshwar University, Solan, Himachal Pradesh

*Corresponding Author E-mail: shailu.bhatt@gmail.com

Online published on 30 April, 2020.

Abstract

A new and simple technique of RP-HPLC was developed and validated for the estimation of Teneligliptin in the tablet dosage form. The partition or separation was accomplished on Kromasil C18 analytical column (150 mm × 4.6 mm, 5.0 μm) utilizing acetonitrile, water and trifluoroacetic acid as mobile phase A and acetonitrile and trifluoroacetic acid as mobile phase B at a flow rate of 1.0 ml/min. The detector used in the present study was UV detector and detection was detected at 245nm. The total chromatographic analysis time per sample was about 55.0 min with teneligliptin eluting at the retention time of about 11.2 minutes. The standard curve was found to be linear in the concentration range of 50–150μg/mL with R2 value of 0.999. The developed method was validated for different parameters like accuracy, precision, specificity and linearity. The developed RP-HPLC method was found to be simple, specific, rapid, reliable and reproducible.

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Keywords

Teneligliptin, HPLC, Assay, Validation.

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