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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 4
First page : ( 1767) Last page : ( 1773)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00319.4

In vitro Antioxidant Capacity of Peperomia pellucida (L.) Kunth Plant from two different locations in Malaysia using different Solvents Extraction

Amirah S1, Zain Haniza H. Mohd1,*, Husni I1, Kassim Nur K.2, Amin I3

1Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Malaysia

2Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

3Department of Nutrition and Dietetics, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

*Corresponding Author E-mail: haniza@fsmt.upsi.edu.my

Online published on 30 April, 2020.

Abstract

The study was performed to investigate the antioxidant capacities of Peperomia pellucida (L.) collected from two different locations using different solvents extraction. Antioxidant capacity was analyzed by 1, 1-Diphenyl-2-picryl hydrazyl (DPPH), β-carotene bleaching and oxygen radical absorbance capacity (ORAC) assays. The ability to scavenge free radical in DPPH assay showed that methanol extract of Penang (ground) sample depicted the highest value of 1108 ± 5.568 mg/ml compared to the other samples. In β-carotene bleaching assay, the ethyl acetate extract of Penang (ground) sample offered the highest percentage of scavenging activity from free radical (86.111%). In ORAC assay, methanol extract of Penang (ground) sample showed the highest value 397.691 μmol of Trolox equivalent/μl. Results between two locations showed no significant difference as compared to the samples from Selangor except for DPPH scavenging assay which depicted a highly significant difference (p<0.05) in Penang. Meanwhile, there was a significant difference (p<0.05) between hexane, chloroform, ethyl acetate and methanol extract of DPPH scavenging, β-carotene bleaching and ORAC assay. Findings showed that ethyl acetate and methanol extracts exhibited a good solvent for antioxidant capacity of P. pellucida. Thus, P. pellucida could be a potential source of antioxidant substance.

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Keywords

Peperomia pellucida, Antioxidant Capacity, DPPH, β-carotene bleaching, ORAC.

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