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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 3
First page : ( 1219) Last page : ( 1223)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00224.3

In vitro Anti-arthritic activity of Methanolic leaf extract of Cadaba indica Lam

Thirumalai V.1, Nirmala P.2,*, Venkatanarayanan R.3

1Department of Pharmacology, Rajah Muthiah Medical College, Annamalai University, Chidambaram

2Professor and Head Department of Pharmacology, Rajah Muthiah Medical College, Annamalai University, Chidambaram

3Principal, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore

*Corresponding Author E-mail: tvthiru90@gmail.com

Online published on 22 April, 2020.

Abstract

Two different types of methanolic leaf extracts of Cadaba indica Lam were studied to prove their anti-arthritic activity. Cadaba indica is a traditional medicinal plant used in folklore for analgesic, anti-inflammatory and antimicrobial actions. In this study, Cadaba indica leaves extract was obtained by hot percolation and cold maceration methods. The Invitro anti-arthritic activity of hot percolation methanolic leaf extract (CIHME) and cold maceration methanolic extract (CICME) were studied by ‘anti proteinase’ and ‘protein denaturation inhibition’ assays. Both extracts were then compared with the standard drug dexamethasone. Different concentrations (100, 200, 400, 800 and 1000μg/ml) of the extract and that of the standard drug were prepared and were analyzed. The results showed that both CICME and CIHME extract effectively inhibited protein denaturation and proteinase enzyme activity in a dose dependent manner. Actions of both extracts were statistically significant when compared with the standard drug dexamethasone, although CICME resulted in a greater ‘percentage inhibition’ than CIHME. Dunnet's‘t’ test was used for data analysis. Our study showed that CICME was more effective and more potent than CIHME. This may be due to a high concentration of bioactive compounds like quercetin, gallic acid and rutin in CICME.

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Keywords

Cold maceration, hot percolation, Anti proteinase activity, Inhibition of protein denaturation, Dexamethasone.

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