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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 3
First page : ( 1121) Last page : ( 1126)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00206.1

Antimicrobial Activity of Streptomyces sp. Isolated from Acidic Peatlands against Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli

Nawan1,*, Isnaeni2, Wasito Eddy B.3

1Department of Microbiology, Faculty of Medicine, Palangka Raya University, Jl. H. Timang Kompleks Tunjung Nyaho, 73111, Palangka Raya, Indonesia.

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, Surabaya, 60286, Indonesia.

3Department of Microbiology Faculty of Medicine, Airlangga University, Jl. Prof. Mustopo, Surabaya, 60286, Indonesia

*Corresponding Author E-mail: dr_na1tanan@yahoo.com; nawan@med.upr.ac.id

Online published on 22 April, 2020.


The incidence of infection by extended spectrum of ß lactamase (ESBL)-producing bacteria is increasing throughout the world. Surveillance results in Indonesia (2012) showed that extended spectrum of ß lactamase (ESBL) producing Escherichia coli was increased (52%). Streptomyces has become an important source of important bioactive compounds with high commercial value and continues to be routinely screened to look for new bioactive substances. The aims of this research is to screen in vitro antibacterial activity of the free cell fermentation Streptomyces sp. of Kalimantan acidic peatland (isolate 2.1). Streptomyces sp. have been isolated from Kalimantan Tengah peat soil (Palangka Raya). The isolatation and fermentation process to obtain antibaterial substances were performed in the International Streptomyces Projects (ISP)-4 media on rotary shaker at 150 rpm, 28C for 5 days. In vitro antibacterial testing of one to 5 days free cell fermentation broth (FCFB) of the Strepromyces sp. isolate 2.1 have been carried out by diffusion agar method. Antibacterial testing using the extended spectrum of ß lactamase (ESBL) Escherichia coli isolated from Dr. Soetomo Hospital patients in Surabaya as test microorganisms. The results of this research is the FCFB of Streptomyces sp. isolate 2.1 showed its activities againts ESBL producing Escherichia coli 6024 and 6110 with inhibition zone average 12, 42 mm and 13, 17 mm. Conclusion of this research is Streptomyces sp. isolated Kalimantan acidic peatland (isolate 2.1) potent to againts ESBL producing Escherichia coli.



Streptomyces sp, free cell fermentation broth, ESBL producing Escherichia coli, diffusion agar method, inhibition zone.


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