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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 1028) Last page : ( 1034)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00189.4

Review on Hyphenation in HPTLC-MS

Bhole R. P.*, Jagtap S. R., Chadar K. B., Zambare Y. B.

Department of Pharmaceutical Quality Assurance, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411018, Maharashtra, India

*Corresponding Author E-mail: ritesh.edu@gmail.com

Online published on 9 April, 2020.


TLC-MS is a versatile technique for separation as well as identification of Pharmaceuticals and Phytopharmaceuticals. Traditionally the separation was carried out by TLC/HPTLC then the separated materials was removed and then identified by Mass spectrometry. Many hyphenated techniques like HPTLC-MS, GC-MS, HPLC-MS, MS-MS Etc. are available. HPLC-MS is more preferred method for separation and identification of compounds. But disadvantage of HPLC is that it requires more solvent as compared to HPTLC. This technique provides efficient, quick and simple method for identification and separation of Narcotic drugs and psychotropic substances. Therefore taking advantage of less solvent requirement in HPTLC and also to enhance working hyphenation of both HPTLC and MS is done so as to provide wide scope for separation as well as identification of product within short period of time. The great advantage of the instrument is that exclusively questioned zones are transferred into the MS for identification and that within less than one-minute sensitive mass spectrometric information is available. This review includes Introduction of method, information of HPTLC and ms, interface for HPTLC-MS, Advantage with example and Applications of method.



HPTLC, Mass Spectrometry, Hyphenation, Interface technique, HPTLC-MS Coupling. Stability indicating assay, isolation of degradation product identification.


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