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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 7
First page : ( 3487) Last page : ( 3490)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00592.4

L-asparaginase from Soil actinomycetes: Optimization of process variables using Plackett Burman Method

Kumar Mithilesh1, Muralidharan Anuraag1, Pai Aravinda2, Subrahmanyam V M1, Kamath Venkatesh1,*

1Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India

2Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India

*Corresponding Author E-mail: kamathvenkatesh82@gmail.com, Venkatesh.kamath@manipal.edu

Online published on 24 December, 2019.

Abstract

Extensive exploration of soil microorganisms in search of novel metabolites have edified researchers on the available natural products for drug discovery. Actinomycetes are filamentous Gram positive bacteria capable of producing wide array of bioactive metabolites. High GC content in the DNA, makes the organism unique and peculiar from other microorganisms. The present article contains data on purification and characterization of Lasparaginase from soil actinomycetes. Soil samples were collected from different locations in Udupi fish market, Karnataka, India. A total of 106 isolates were screened through rapid plate assay method, out of which 17 were found active. Furthermore, a promising isolate FMCL-13A with maximum enzyme activity was chosen by submerged fermentation technique. Statistical designs viz., Placket-Burman (PB) was employed for optimization of various experimental parameters in order to enhance L-asparaginase production. The nutritional variables which affects the enzyme production by submerged fermentation from the active isolate was optimized by PB model.

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Keywords

Actinomycetes, L-asparaginase, placket burman model, optimization.

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