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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 6
First page : ( 2987) Last page : ( 2994)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00505.5

Analysis of Antimicrobial Activity of Woodfordia fruticosa, Adhatoda vasica and Ricinus communis Against Multi-Drug Resistant Bacteria

Joshi Mahavir1, Kaur Sukhminderjit1, Verma Monika1, Mishra Tulika2,*

1University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413, India

2School of Medicine, Xavier University, Santa Helenastraat 23, Oranjestad, Aruba, Dutch Caribbean

*Corresponding Author E-mail: tulikares@gmail.com

Online published on 8 August, 2019.

Abstract

Introduction

Emergence of antibiotic resistance bacteria is one of major global problem and researcher are all focused to treat infection caused by antibiotic bacteria. Plants are used for their medicinal property from the ancient time. Plants are rich source of many phytoconstituents that do not possess any side effect.

Material and methods

For the present work multidrug resistance bacteria were isolated from hospital soil sample and checked for antibiotic resistance by using antibiotic disk. The microorganisms showing resistant against more than three antibiotics were selected and identified by sequencing 16sRNA sequencing. Leaves extract of Woodfordia fruticosa, Adhatoda vasica and Ricinus communis plant prepared in ethanol, methanol and aqueous solvents were tested against for antimicrobial activity.

Results

Two multidrug resistance bacteria Kingella Sp. and Klebsiella sp. showed antibiotic resistant against more than three antibiotics sequenced for 16sRNA same compared and identified by phylogenetic tree analysis using MEGA 5.2 software. Methanolic extract of all three plant leaves showed comparatively good antimicrobial activity against two clinical isolates.

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Keywords

Antimicrobial activity, Woodfordia fruticosaAdhatoda vasicaRicinus communis, clinical Isolates, Kingella Sp.Klebsiella sp..

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