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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 6
First page : ( 2902) Last page : ( 2906)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00489.X

The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line

Hanifah Ratu Shafira1, Novitarani Nadia Aida1, Harmen Fadhilah1, Tedjo Aryo2,3,*, Azizah Norma Nur2,3, Putrianingsih Rista2,3, Fachri Wilzar2,3, Kusmardi Kusmardi3,4

1Medical Student, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia

2Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia

3Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia

4Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia

*Corresponding Author E-mail: aryo.tedjo@gmail.com

Online published on 8 August, 2019.

Abstract

Objective of this research to determine the inhibition of ethanol extract of Phaleria macrocarpa Boerl. stem bark on COX-2 expression in colorectal cancer cell line HCT116. Ethanol extract of Phaleria macrocarpa stem bark was prepared for maceration in ethanol. Phaleria macrocarpa stem bark in-vitro anticancer activity was tested with MTT assay on HCT116 colorectal cancer cell line. This study also assessed the effect of ethanol extract of Phaleria macrocarpa stem bark on the inhibition of COX-2 expression in the HCT116 colorectal cancer cell line by counting the H-score from immunocytochemistry staining with help from ImageJ and TMARKER application. Ethanol extract of Phaleria macrocarpa stem bark shows anticancer activity by inhibiting its growth with IC50 of 1, 327 μg/ml. One of the pathways is through inhibition of COX-2 expression, shown by the H-score of 173, 33 after administration of Phaleria macrocarpa stem bark at a dose of 200 ppm. This study shows that Phaleria macrocarpa stem bark is a colorectal cancer growth inhibitor, one of which is through inhibition of COX-2.

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Keywords

COX-2, colorectal cancer, IC50, immunocytochemistry, MTT assay, Phaleria macrocarpa.

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