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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 6
First page : ( 2748) Last page : ( 2752)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00460.8

Evaluation of Anticancer activity of Ethanol extract of Bauhinia tomentosa Linn. on A549, Human Lung Carcinoma Cell lines

Balabhaskar R1,*, Vijayalakshmi K2

1Research Scholar, Department of Biochemistry, Bharathiar University. Coimbatore-641046

2Associate Professor, Department of Biochemistry, Bharathi Women's College, Chennai-600108

*Corresponding Author E-mail: rbalabhaskar@gmail.com

Online published on 8 August, 2019.

Abstract

Objective

The aim of the present study is to evaluate the anticancer activity of ethanol extract of B.tomentosa (EBT).

Methods

The anticancer activity of ethanol extract of B.tomentosa was evaluated by MTT Assay, AO/EtBr Fluorescence assay and DNA Fragmentation assay against A549 (human lung cancer cell line).

Results

Treatment of A549 cells with EBT (at IC50 concentration) for 48 hrs significantly inhibited the proliferation in a concentration dependent manner. AO/EtBr staining confirms the occurrence of apoptosis in A549 cells after the treatment of EBT. From DNA fragmentation assay, it is evident that EBT has also significantly induced DNA damage in treated A549 cells.

Conclusion

Thus the present study demonstrates the anticancer activity of EBT. The ethanol leaf extract of B.tomentosa has shown potent anticancer activity on A549 cancerous cell line. Further studies are needed to identify the bioactive molecules and to explore its mechanism of action which might throw light in the development of new alternative drug for lung cancer.

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Keywords

Anticancer activity, B.tomentosa, MTT assay, AO/Et Br, DNA Fragmentation, A549.

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