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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 5
First page : ( 2391) Last page : ( 2395)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00400.1

In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7

Fadilah Fadilah1,4,*, Wiyono Lowilius2, Edina Brenda Cristie2, Rahmawati Risya Amelia2, Erlina Linda1,4, Tedjo Aryo1,3, Paramita Rafika Indah1,4

1 Departement of Medical Chemistry, Faculty of Medicine, University of Indonesia

2Medical Student of Faculty of Medicine, University of Indonesia

3Researcher of Drug Development Research Center, Indonesian Medical Education and Research Institute, Faculty of Medicine, University of Indonesia

4Researcher of Bigdata and Bioinformatics Research Center, Indonesian Medical Education and Research Institute, Faculty of Medicine, University of Indonesia

*Corresponding Author E-mail: fadilah81@gmail.com

Online published on 8 August, 2019.

Abstract

Breast cancer most commonly occurs in women worldwide, in 2012 there were 1.670.000 cases with the second most prevalence in the world (25% of the total incidence of cancer). Drugs used so far have not been effective and many resistance occurs, it is necessary to a discovery of herbs that contain polyphenols as anticancer. Kaempferia pandurata Roxb plant has been identified to contain various essential oils and several flavonoid compounds that have demonstrated many biological activities. This research was done by simulation study with molecular docking approach which then extraction, the result of the extract was tested in vitro activity against Breast Cancer Cell Line MCF-7. The docking results show that the flavonoid compound against the target ERbreast cancer has an enthalpy energy ΔG-11.75 kcal/mol, the phytochemical results support that the flavonoids compound dominates the composition of the Kaempferia pandurata Roxb. The Result of in vivo test with MTT method IC50 value of standard cisplatin, hexane extract was 8.42; 26.70 μg/ml respectively to MCF-7 cell line showed that Kaempferia pandurata Roxb extract very potential as an antibreast cancer.

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Keywords

Molecular docking, Phytochemical test, Antibreast cancer, Kaempferia pandurata Roxb, MCF-7 cell line.

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