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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 4
First page : ( 1573) Last page : ( 1579)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00261.0

The Efficacy of Human Dental Pulp Stem Cells in regenerating Submandibular Gland Defects in Diabetic Wistar Rats (Rattus novergicus)

Suciadi Septiana P.1, Nugraha Alexander P.2,3,4, Ernawati Diah S.5,*, Ayuningtyas Nurina F.5, Narmada Ida B.2, Prahasanti Chiquita6, Dinaryanti Aristika4, Ihsan Igo Syaiful4, Hendrinto Eryk4, Susilowati Helen4, Rantam Fedik Abdul4,7

1Faculty of Dentistry, Universitas Airlangga, Surabaya, Indonesia

2Orthodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

3Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

4Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia

5Oral Medicine Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

6Periodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

7Virology and Immunology Laboratory Microbiology Department Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia

*Corresponding Author E-mail: diah-s-e@fkg.unair.ac.id

Online published on 8 August, 2019.

Abstract

Chronic hyperglicemia in Diabetes Mellitus caused microangiopathy in salivary gland. Human Dental Pulp Stem Cells (HDPSCs) suspected can regenerate the defect. The aim of this study was to analyze the efficacy of HDPSCs in stimulating angiogenesis, acinar cell numbers and Transforming Growth Factor-β (TGF-β) serum to regenerate submandibular gland defects in diabetic Wistar rats. Twenty-four male Wistar (250–350 g) rats 3-months-old were used. Rats were divided into 4 groups (n=6 each: a positive control group on Day 7 (DM) (C+7), a positive control group on Day 14 (DM) (C+14), a treatment group on Day 7 (DM+5.105 HDPSCs transplantation intraglandular) (T7) and a treatment group on Day 14 (DM+5.105 HDPSCs transplantation intraglandular) (T14). Wistar Rats were administered with 30 mg of Streptozotocin per kg of bodyweight to induce Diabetes Mellitus (DM). Histopathological examination with HE staining was performed to analyse neovascularization and acinar cell numbers. ELISA was performed to measure TGF-β serum. Statistical analysis used: A Tukey HSD or Bonferroni test after ANOVA or Kruskal Wallis test was performed (p<0.05) based on a Saphiro Wilk and Levene's test (p>0.05). The highest acinar cell number was found in the T7 group [513.167±136.17] with no significant difference [p=0.136, p<0.05]. The highest capillaries were found in T14 [10.667±4.54] and TGF-β serum level [168.87±37.38] with significant difference [p=0.006; p<0.05] and [p=0.008, p<0.05]. HDPSCs can regenerate submandibular gland defects in Diabetic Wistar rats by stimulating angiogenesis, acinar cells number and TGF-β serum.

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Keywords

Human Dental Pulp Stem Cells, Submandibular Gland Defect, Diabetes Mellitus, Acinar Cells, Angiogenesis, Transforming Growth Factor-β.

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