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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 2
First page : ( 757) Last page : ( 763)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00134.3

Poly Herbal Hair Oil Preparation, Standardization, Treatment and Evaluation for Alopecia in Male Wistar Rats

Nikita Saraswat1,*, Rashmi Pal S1, Yogendra Pal1, Pranay Wal2, Ankita Wal2, Rai A.K.3

1Assistant Professor, Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur

2Associate Professor, Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur

3Professor, Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur

*Corresponding Author E-mail: nikita.saraswat07@gmail.com

Online published on 18 April, 2019.



Baldness is very common in males and females but predominantly it is seen more in males. This accounts for approximately 89–99 percent of hair loss. Though there are multiple causes responsible for causing baldness like hormones, hereditary, age factors, pollution, work environment, stress and many more.


The objective of the paper is to evaluate the effectiveness of poly herbal hair oil prepared in the laboratory from sources such as hibiscus, leech, beehive, and onion which could be a potential hair growth promoter.


The hair from the dorsal area of male wistar rats was shaved and they were divided into three groups having 6 rats each. Group I received the application of coconut oil daily, Group II received application of Minodixil (2%) ethanol extract as standard hair growth promoter and Group III received the application of poly-herbal oil prepared in the laboratory. The oil in respective groups was applied daily on rats of each group on the shaved surface.


The initiation of hair growth in all groups was calculated as a mean value to compare the results in different groups. Then the length of hair was noted on 15, 21, 30 and 40th day in centimeters. The mean length was calculated on each day for every group and the results were compared. The anagen/telogen ratio was also calculated for all the groups for better evaluation of results.


The hair growth was maximum in the case of Group III, on which the poly herbal formulation of oil was applied daily. The hair growth in Group II (Minoxidil) was observed with optimum hair growth while the Group I was observed with least hair growth after the final evaluation and comparison of all the results. A significant hair growth effect from the poly herbal formulation oil was noted from the research conducted.



Alopecia, Leech oil, Hair Loss, Baldness, Poly herbal hair oil, herbal extract.


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