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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 12
First page : ( 5811) Last page : ( 5814)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.01006.0

Simultaneous Equation Method for Estimation of Rutin Trihydrate and Berberine Chloride in Synthetic Mixture and Polyherbal Formulation

Dr Chaudhary Palak*, Dr. Patel Harsha U.

Shri Satsangi Saketdham “Ram Ashram”, Group of Institutions, Faculty of Pharmacy, Vadasma, Mehsana, Gujarat, India

*Corresponding Author E-mail: palakc24@gmail.com

Online published on 24 February, 2020.


The present study was focused on establishing and validating a simple, accurate, reliable and economical UV spectrophotometric method for the simultaneous determination of Rutin trihydrate (RUT) and Berberine Chloride (BER) in synthetic mixture and in antidiabetic polyherbal formulation. Rutin and Berberine from herbal plants are used as antidiabetic drug to prevent and manage Diabetes. The developed method gives best results in terms of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The method was validated as per ICH guidelines. The linearity range for Rutin trihydrate and Berberine Chloride were found tobe 10 to50 μg/ml and 10 to 50 μg/ml respectively. The values of LOD were 0.062 μg/ml and 0.076 μg/ml and the values of LOQ were found to be 0.187 μg/ml and 0.229 μg/ml for Rutin trihydrate and Berberine chloride respectively.



Rutin trihydrate (RUT), Berberine chloride (BER), Simultaneous equation method, Validation, Polyherbal formulation.


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