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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 11
First page : ( 5467) Last page : ( 5471)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00948.X

Evaluation of hepatoprotective and anticancer potential of Ehretia laevis: An In-vitro evidence

Rangnathrao Tarke Santosh1,*, Shanmugasundaram P.2

1Department of pharmaceutical Chemistry and Analysis, School of Pharmaceutical Sciences, VELS Institute of Science, Technology and Advances Studies (VISTAS), Chennai-600117

2Director, VELS college of Pharmacy, VELS University, Chennai-600117

*Corresponding Author E-mail: tarkesantosh@gmail.com

Online published on 9 April, 2020.

Abstract

Background

Ehretia laevis has been used to treat Jaundice in various Uttarakhand sub-Himalayan regions in India. The current study focused primarily on Ehretia laevis hepatoprotective and anticancer activity.

Objective

The present study validates Ehretia Laevis traditional claim as a jaundice remedy by evaluating in vitro hepatoprotective properties of Ehretia laevis leaves along with their anti-cancer properties.

Materials and Methods

The well-known model of carbon tetrachloride induced in vitro rat hepatocyte injury in HepG2 cells, the hepatoprotective effect of the hydroalcoholic extract, ethyl acetate fraction and aqueous fraction of Ehretia laevis was evaluated. Further the concentrations of aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), triglycerides (TGL) and total proteins in the culture medium were evaluated as an indication of hepatocytes necrosis using Ecoline diagnostic kits. The MTT assay was used to estimate the in vitro anticancer activities of the hydroalcoholic extract, ethyl acetate fraction and aqueous fraction of Ehretia laevis against cancer cell lines [Vero (African, Green Monkey Kidney), MCF-7 (Human, Breast Carcinoma), A-549 (Human, Lung Carcinoma) HEP-G2 (Human, Liver Carcinoma)].

Result

Among the extracts evaluated ethyl acetate fraction showed significant Hepatoprotective and anticancer activities at low concentration. Ehretia laevis demonstrated dose-dependent hepatoprotective activity.

Conclusion

The present study validates its traditional use as a remedy of jaundice and can provide promising therapeutic interventions against chemical-induced liver damage. Moreover Ehretia laevis also showed significant anti-cancer activity.

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Keywords

Anticancer, Ehretia Laevis, Hepatoprotective, MTT, Cell line.

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