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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 11
First page : ( 5315) Last page : ( 5319)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00921.1

Detection of Variation in Expression of Insecticide Resistance Gene Cyp4d2 involved in Detoxification of Insecticides in Drosophila melanogaster

Hembrom Preety Sweta, Jose Jisna, K Shalini, Grace Tony*

Department of Genomic Science, School of Biological Sciences, Central University of Kerala, Padanakkad, Kasaragod, Kerala, India

*Corresponding Author E-mail: tonygrace99@gmail.com

Online published on 9 April, 2020.


Insecticides which are used to control insects by killing them or restricting them from executing destructive behaviors are one of the major factors for improving the agricultural yield and preventing crop destruction. However, widespread and overuse of chemical insecticides has led to many harmful effects on the environment as well as on mankind. Frequent use of the same group of chemical insecticides has resulted in the evolution of insecticide resistance. Insecticide resistance develops by the selection of genes that provide tolerance to these chemicals. The present study aims to determine if CYP4d2 gene has any role in endosulfan detoxification. The expression analysis of CYP4d2 gene using Real Time PCR revealed that CYP4d2 exhibited a significant upregulation in the Periya sample, whereas downregulation in the Muliyar sample. Higher expression of CYP4d2 gene was observed in the endosulfan-treated adult stage when compared to the endosulfan-treated larval stage.



CYP4d2, Cytochrome p450, Detoxification, Endosulfan, Gene Expression, Insecticides, RT-PCR.


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