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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 11
First page : ( 5265) Last page : ( 5268)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00911.9

The Effect of Omega-3-enriched Fish oil on the Inflammation of Mice Colon Induced by AOM and DSS: Study on COX-2

Kusmardi Kusmardi1, Adare Pater Dean2,3,*, Kodariah Ria1

1Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia

2Postgraduate Study Program of Biomedic, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia

3Faculty of Medicine, University of Papua, Papua, Indonesia

*Corresponding Author E-mail: paterdean@gmail.com

Online published on 9 April, 2020.



Colorectal cancer is one of the leading causes of cancer deaths in some countries of the world. Inflammation has been recognized as one of the etiologies of colorectal cancer. Omega-3 is known to have anti-inflammatory properties. The aim of this tudy was to investigate the anti-inflammatory effect of omega-3-enriched-fish oil on inflammation of mice's colon by induction of azoxymethane (AOM) and dextran sodium sulfate (DSS) by analyzing the expression of cyclooxygenase-2 (COX-2).


30 Swiss Webster mice divided into 6 groups: (1) Negative control group (KN); (2) test group of fish oil with a dose of 1.5 mg/day (Dose-I); (3) test group of fish oil with a dose of 3 mg/day (Dose-II); (4) solvent-control group of corn oil (KP); (5) Positive control group of aspirin (ASP); (6) Normal group (KNO). Group 1–5 was induced with AOM 10 mg/kgBB intraperitoneally once, then 1 week later followed by administering 2% ad lib DSS in drinking water for 7 days. After the induction period, continued treatment time based on group above for 6 weeks.


There were no significant diffenrence in the test group (Dosis-I dan Dosis-II) (p>0.05) related to COX-2 compared with all control groups. Significant differences in the expression of COX-2 were found by comparing all treatment groups with the normal group (p <0.05).


omega-3-rich fish oils with dose of 3 mg and 1.5 mg were not able to inhibit inflammatory proces of AOM+DSS-induced colon mice.



AOM, COX-2, DSS, Fish oil, omega-3, inflammatory.


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