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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 11
First page : ( 5247) Last page : ( 5250)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00907.7

Identification of Secondary Metabolite using Phytochemical and Infra-Radiation Test on the Leaves of Vitex pinnata found in the Seulawah Agam mountain region of Aceh

Nuraskin Cut Aja1,5,*, Marlina2, Idroes Rinaldi2, Soraya Cut3, Djufri4

1Program Studi Doktor Matematika dan Aplikasi Sain, Universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh, 23111, Indonesia

2Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh, 23111, Indonesia

3Fakultas Kedokteran Gigi, universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh, 23111, Indonesia

4Biology Education Department Faculty of Teacher Training and Education, Syiah Kuala University, Kopelma Darussalam, Banda Aceh, 23111, Indonesia

5Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, DarulImarah, Lheu Blang, Banda Aceh, Kabupaten Aceh Besar, Aceh, 23231, Indonesia

*Corresponding Author E-mail: canuraskin@yahoo.com

Online published on 9 April, 2020.

Abstract

Laban (Vitex pinnata) is a member of the verse majority of plants used by people around the world to treat diseases. The leaves of this plant are used to treat fever and hypertension; the stem bark to treat stomach pain and diarrhea, while the root skin is used as a tea that can relieve fatigue and body aches. This research study was conducted to identify the active compound of the laban leaf and to also carry out a Fourier Transform Infrared Radiation (FTIR) test on it. This study was done on the n-hexane, ethyl acetate, and methanol solvent extracts of laban leaves. The extracts were then concentrated and the quality of the extracts assessed using a phytochemical test and FTIR test. After the extraction process, the qualities of the extract of n-hexane from laban leaves using an organoleptic test was brown to black-brown in color, and had a herbaceous odor. The weight of the extract was 59.82g, with a yield value of 1.994%. The ethyl acetate extract was assessed using an organoleptic test. It had a dark black color and a typical henna smell. The extract had a weight of 189.089g and a yield value of 6.30%. Using an organoleptic test to assess the quality of the methanol extract, a typical henna smell was noticed and the extract color was dark black. The weight of the extract was 442.06g and it had a yield value of 14.73%. A secondary metabolite test obtained data that showed that the n-hexane extract was positive for steroids and phenol/tannin, while ethyl acetate extract was positive for steroid, terpenoid, saponin, flavonoid and phenol/tannin using the Dragendorff's and Wagner's reagents. The methanol extract was positive for steroid, terpenoid, saponin, flavonoid and phenol/tannin using Mayer's and Dragendorff's reagents. The extracts of n-hexane, ethyl acetate and methanol were then electrolyzed with Fourier Transform Infrared Radiation (FTIR) to see the constituents of the extracts at different wavelengths for each functional group.

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Keywords

Laban leaf (Vitex pinnata), secondary metabolite identification, FTIR analysis.

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